Giovanni Ambrogio de Predis (c. 1455 – c. 1508) was an Italian Renaissance painter from Milan, who is known for having collaborated with Leonardo da Vinci, and with his own brother Evangelista, in the painting of the Virgin of the Rocks for the altarpiece in the chapel of the Confraternity of the Immaculate Conception at the Church of San Francesco Grande, Milan. Ambrogio, who was born in Preda, gained a reputation as a portraitist, including as a painter of miniatures, for the court of Ludovico Sforza.
A marriage was arranged between Emperor Maximilian I and Bianca Maria Sforza, niece of Ludovico il Moro, but before the former would commit to the arrangement, he requested a portrait of his proposed bride. The portrait of Bianca Maria was painted by Ambrogio, who followed her to Innsbruck after the wedding in 1493. In 1494 he returned to Milan, where he designed coins for the mint, designed and supervised tapestry works, and prepared stage scenery. In 1502 he produced his only surviving signed and dated work, a portrait of the Emperor Maximilian. Much of Ambrogio de Predis's artistic output remains in dispute. The side panels for the Virgin of the Rocks, now in the National Gallery, London were stated by the brothers to have been painted by them during the legal dispute over the altarpiece, and this is accepted by art historians.
A Portrait of Youth as Saint Sebastian at the Cleveland Art Museum is attributed to Abmbrogio de Predis.

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